cd4 t lymphocytes  (Bio-Rad)

Journal: Frontiers in Immunology

Article Title: Canine diabetes mellitus demonstrates multiple markers of chronic inflammation including Th40 cell increases and elevated systemic-immune inflammation index, consistent with autoimmune dysregulation

doi: 10.3389/fimmu.2023.1319947

Figure Lengend Snippet: Th40 cell comparison in CD and HC. PBMC analyzed for CD3, CD4 and CD40 (Th40 cells). (A) Healthy controls and (B) Canine Diabetes were analyzed by dot plots to establish forward versus side scatter. Within the FSC x SSC population CD3+ cells are determined above background controls (grey solid histogram). Within the gated CD3+ population, contour plots of CD4 versus CD40 were determined. Th40 cells are defined as CD3+ then CD4+CD40+/total CD4+ cells (Q2/Q2 + Q3). (C) Th40 percentage in CD dogs compared to HC dogs. Statistics were done using Graph Pad Prism using a two-tailed, unpaired t-test analysis.

Article Snippet: Staining antibodies included: anti-CD3 (FITC) from Bio-Rad, cat# MCA1774F; anti-CD4 (APC) from Bio-Rad, cat#MCA1038APC; anti-CD40 (G28-5; Alexafluor 405) produced in house; there is no canine anti-CD40 antibody available so the G28-5 anti-CD40 that recognizes human CD40 was used.

Techniques: Comparison, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Canine diabetes mellitus demonstrates multiple markers of chronic inflammation including Th40 cell increases and elevated systemic-immune inflammation index, consistent with autoimmune dysregulation

doi: 10.3389/fimmu.2023.1319947

Figure Lengend Snippet: Inflammatory cytokine levels in CDM Th40 cells. PBMC from nine CDM and five healthy controls were analyzed. (A) Cells were stained for CD3 then gated against CD4 and CD40; dot plot is representative of CDM. Within the Th40 (CD4+CD40+) and Conventional T cell (CD4+CD40-) populations, intracellular levels of (B) IL-6 and (C) IFNγ were determined. Statistics were done from GraphPad Prism using a two-tailed, unpaired t-test analysis.

Article Snippet: Staining antibodies included: anti-CD3 (FITC) from Bio-Rad, cat# MCA1774F; anti-CD4 (APC) from Bio-Rad, cat#MCA1038APC; anti-CD40 (G28-5; Alexafluor 405) produced in house; there is no canine anti-CD40 antibody available so the G28-5 anti-CD40 that recognizes human CD40 was used.

Techniques: Staining, Two Tailed Test

Journal: PLoS ONE

Article Title: Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer

doi: 10.1371/journal.pone.0033274

Figure Lengend Snippet: Facs sorted CD11b + CD14 − MHCII − cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4 + and CD8 + T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3 H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

Article Snippet: For T cell isolation, PBMCs were isolated as previously described from fresh blood samples of healthy dogs and stained with 0.3 μg of FITC-conjugated mouse anti-dog CD3 (clone CA17.2A12, AbD Serotec), 0.15 μg of Pacific blue-conjugated mouse anti-dog CD4 (clone YKIX302.9, AbD Serotec) and 0.15 μg of Alexa700-conjugated mouse anti-dog CD8 (clone YCATE55.9, AbD Serotec) antibodies.

Techniques: Isolation, Incubation, Negative Control